Automated DNA/RNA Sizing and Quantitation Overview

Determination of the size, concentration and quality of DNA and RNA is done nowadays on automated electrophoresis instruments, which significantly increase the number of samples that can be processed at a time and produce a normalized digital output that can be used for comparisons within a run or between various runs.

TAGC currently has two automated DNA/RNA Electrophoresis instruments:

 

Qiagen QIAxcel  is capable of analyzing up to 96 DNA samples at a time in a 12-strip tube or 96-well plate format, report the results as a gel image and electropherogram and determine the size and the concentration of the DNA fragments in each sample. 

TAGC provides initial QIAxcel training at $80 and charges $8 for each subsequent 12-sample run.

During the training session, the user sets up a 12-sample run and analyses the data with the help of a member of the TAGC staff. Upon completion of the required training, the users are free to book and run the instrument during TAGC hours.

 

Agilent Bioanalyzer 2100 is a chip based electrophoresis method capable of running 11-12 DNA or RNA samples at a time and is customarily used for analysis of Next Generation Sequencing Libraries amongst other types of material.  

 The kits currently offered are:

  • DNA 1000 chip: $ 48.00
  • High Sensitivity chip: $75.00

TAGC provides intial Bioanalyzer training for $80, during which the user runs a chip of their choice and analyzes the data under supervision of a TAGC staff member. Upon completion of the required training, the users are free to book and run the instrument during TAGC hours.

 

To find out which instrument and technology best suits your research needs contact TAGC.

 

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