Next Generation Sequencing (NGS) consists of high throughput sequencing of thousands to millions of DNA molecules in parallel.
Primarily, the procedure starts by acquisition of template material consisting of DNA or RNA/cDNA. Then, if desired, regions of interest are enriched from acquired template pool by DNA target capture and/or amplification using various types of PCR. Next, the desired DNA sample is used to construct a DNA library with additional modifications necessary for NGS, such as addition of 5’ and 3’ DNA adapters. The adapter ligated libraries are then clonally amplified and sequenced by various means depending on the platform.