Sanger Sequencing Overview

TAGC offers Sanger sequencing services at $4.75/sample for all researchers within the University of Alberta and at $6.00/sample for all  external users. These prices are effective Feb 09, 2015.

The facility houses one 3130 (4-capillary) and one 3130xl (16-capillary) Life Technologies Genetic Analyzers and has the capacity to process 192 samples per day.

We use BigDye Terminator v3.1 Cycle Sequencing Kit and Agencourt CleanSEQ, a magnetic beads based post-reaction clean up protocol, automated on a Biomek 3000 work station.

We offer assistance with data analysis and protocol troubleshooting.

 

Next Sanger Sequencing Method