Delayed signal with no base calling



Delayed - raw -1-website- 2

Delayed - raw -2-website - 3

The read start point, normally around 1,900, is more or less delayed.


Delayed - electropherogram-website-

Broad, overlapping peaks, with no base calling.



Possible causes


Template concentration too high

Quantify the DNA template.¬†Ensure that the concentration is in the range specified on the “Sample Preparation and Submission” TAGC web page.

Template contains a high amount of salts, which get preferentially injected

Clean up template.