Relatively even raw signal across the whole length. Gradual drop is normal.
Signal intensity around 1,000 RFUs. Values between 100 and 4,000 RFUs are acceptable.
Beginning of the sequence: good quality reading starting around 30 nucleotides.
Middle of the sequence: sharp, evenly spaced peaks, with a clean, flat baseline.
End of the sequence: increasing broadening of the peaks and lower peak heights past 600, with continued base calling till 900 nucleotides.